DNA Concentration Calculator
Calculate DNA concentration from absorbance readings
Note: This calculator determines DNA concentration using absorbance at 260nm and assesses purity using A260/A280 ratio.
DNA Concentration
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Total DNA Amount
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A260/A280 Ratio
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A260/A230 Ratio
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Purity Assessment
Enter absorbance values to see purity assessment.
Understanding DNA Concentration
- DNA Concentration: Calculated using Beer-Lambert law
- A260/A280 Ratio: Indicates protein contamination (1.8-2.0 is pure)
- A260/A230 Ratio: Indicates organic contamination (>2.0 is clean)
- Extinction Coefficients: dsDNA: 50 μg/mL, ssDNA: 33 μg/mL, Oligo: 20 μg/mL
Quality Control Tips
- Use fresh cuvettes and clean spectrophotometer
- Blank with the same buffer used for dilution
- Measure absorbance in the linear range (0.1-1.0)
- Consider using fluorometric methods for low concentrations
- Validate with gel electrophoresis or qPCR
Important: This calculator provides estimates based on absorbance measurements.
Actual concentrations may vary due to buffer composition, temperature, and instrument calibration.
Always validate with alternative methods for critical applications.