DNA Copy Number Calculator
Calculate DNA concentration, copy numbers, and molecular weight
Note: This calculator determines DNA copy numbers, concentration, and molecular weight based on absorbance measurements and DNA length.
Results
DNA Concentration (ng/μL)
-
DNA Concentration (μg/mL)
-
DNA Concentration (nM)
-
Total DNA Mass (ng)
-
Molecular Weight (g/mol)
-
Copy Number
-
Molecules per μL
-
Formulas Used
DNA Concentration (ng/μL):
[DNA] = A260 × 50 × Dilution Factor × 50
Copy Number:
Copy Number = (Concentration × Volume × 6.022×10²³) / Molecular Weight
Molecular Weight:
MW = DNA Length × 650 g/mol (dsDNA)
MW = DNA Length × 330 g/mol (ssDNA)
MW = DNA Length × 340 g/mol (RNA)
[DNA] = A260 × 50 × Dilution Factor × 50
Copy Number:
Copy Number = (Concentration × Volume × 6.022×10²³) / Molecular Weight
Molecular Weight:
MW = DNA Length × 650 g/mol (dsDNA)
MW = DNA Length × 330 g/mol (ssDNA)
MW = DNA Length × 340 g/mol (RNA)
Important: This calculator assumes pure DNA samples. Contaminants like proteins, RNA, or organic compounds can affect absorbance readings.
For accurate results, ensure your DNA sample is properly purified and use appropriate blank controls.