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DNA Concentration Calculator
Calculate nucleic acid concentration from A260 absorbance
Formula: Concentration = A260 Reading × Factor × Dilution Factor.
(dsDNA Factor = 50 µg/mL. Assumes 1 cm path length).
(dsDNA Factor = 50 µg/mL. Assumes 1 cm path length).
Spectrophotometer Data
Calculation Results
⚠️ This calculator is for laboratory and educational use. Ensure you check your sample's A260/A280 ratio to assess purity (ideal ~1.8 for DNA, ~2.0 for RNA). Contaminants like proteins or phenol can artificially inflate A260 readings.