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Annealing Temperature Calculator
Calculate optimal PCR annealing temperature from primer melting temperatures
Note: Uses the common formula Ta = 0.3×Tm(short) + 0.7×Tm(long) − 14. For Wallace rule Tm: 2(A+T) + 4(G+C) for primers under 20 bp.
Primer Sequences
Results
⚠️ This calculator is for educational purposes only. Always consult a veterinarian, agronomist, or qualified professional before making health, breeding, or agricultural decisions.
About the Annealing Temperature Calculator
Free annealing temperature calculator for PCR. Enter primer sequences or Tm values to find the optimal Ta for successful amplification.