Is this Annealing Temperature Calculator free to use?

Yes, Annealing Temperature Calculator is 100% free to use online with no installation or account required.

How do I use the Annealing Temperature Calculator?

Simply enter your values into the required input fields and click calculate to view the results instantly.

Yes, the calculator is fully responsive and compatible with Android, iOS, Windows, macOS, and Linux web browsers.

Annealing Temperature Calculator — Free Online Tool

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Calculate optimal PCR annealing temperature from primer melting temperatures

Note: Uses the common formula Ta = 0.3×Tm(short) + 0.7×Tm(long) − 14. For Wallace rule Tm: 2(A+T) + 4(G+C) for primers under 20 bp.

Primer Sequences

Primer 1 Sequence (5'→3')

Primer 2 Sequence (5'→3')

⚠️ This calculator is for educational purposes only. Always consult standard protocols or experimental optimization for precise PCR settings.

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