Protein Concentration Calculator
Calculate protein concentration from absorbance readings
Note: This calculator determines protein concentration using various methods including Bradford, BCA, and absorbance at 280nm.
Protein Concentration
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Total Protein Amount
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Molar Concentration
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Method Used
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Quality Assessment
Enter parameters to see quality assessment.
Understanding Protein Concentration
- Bradford Assay: Based on Coomassie Blue binding to proteins
- BCA Assay: Based on copper reduction by protein
- A280 Method: Based on UV absorbance of aromatic amino acids
- Lowry Assay: Based on Folin-Ciocalteu reagent reaction
Measurement Best Practices
- Use fresh reagents and standards
- Include appropriate controls and blanks
- Measure within the linear range of the assay
- Account for interfering substances
- Validate with multiple methods when possible
Important: This calculator provides estimates based on standard curves and theoretical values.
Actual concentrations may vary due to protein composition, buffer effects, and assay conditions.
Always validate with appropriate standards and controls.