qPCR Efficiency Calculator
Calculate PCR efficiency, standard curves, and relative quantification
Note: This calculator determines qPCR efficiency, standard curve parameters, and relative quantification using Ct values and standard concentrations.
Results
PCR Efficiency (%)
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Slope
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Y-Intercept
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R² Value
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Unknown Sample Concentration
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Formulas Used
PCR Efficiency:
Efficiency = (10^(-1/slope) - 1) × 100%
Standard Curve:
Ct = slope × log(concentration) + intercept
Concentration from Ct:
Concentration = 10^((Ct - intercept) / slope)
Ideal Efficiency: 90-110% (slope: -3.1 to -3.6)
Efficiency = (10^(-1/slope) - 1) × 100%
Standard Curve:
Ct = slope × log(concentration) + intercept
Concentration from Ct:
Concentration = 10^((Ct - intercept) / slope)
Ideal Efficiency: 90-110% (slope: -3.1 to -3.6)
Important: For accurate qPCR efficiency calculations, ensure your standard curve has at least 5-6 data points spanning 4-6 orders of magnitude.
Ct values should be in the linear range of your assay. Poor efficiency may indicate primer design issues, reaction conditions, or template quality problems.