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Enzyme Activity Calculator
Calculate volumetric enzyme activity (U/mL) using spectrophotometric assay data
Formula: $\text{Activity (U/mL)} = \frac{(\Delta A / \Delta t) \cdot V_{\text{total}}}{\varepsilon \cdot l \cdot V_{\text{enzyme}}}$
Where $\Delta A / \Delta t$ is absorbance change per minute, $\varepsilon$ is molar extinction coefficient, $l$ is cuvette pathlength, and $V$ are volumes.
Where $\Delta A / \Delta t$ is absorbance change per minute, $\varepsilon$ is molar extinction coefficient, $l$ is cuvette pathlength, and $V$ are volumes.
Assay Parameters
Calculation Results
⚠️ Enzyme activity assays depend critically on optimal temperature, pH, and substrate saturation ($[S] \gg K_m$). Ensure initial linear rates are measured to prevent underestimating activity due to substrate depletion or product inhibition.