Michaelis-Menten Equation Calculator — Free Online Tool
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Michaelis-Menten Equation Calculator

Calculate the initial reaction velocity (v0) of an enzyme

Formula: v0 = (Vmax × [S]) / (Km + [S])
Where Vmax is maximum velocity, [S] is substrate concentration, and Km is the Michaelis constant. Ensure Km and [S] are in the same units.
Enzyme Kinetics Parameters

Calculation Results

⚠️ The Michaelis-Menten equation relies on the steady-state approximation. It does not apply to allosteric enzymes (which show sigmoidal kinetics rather than hyperbolic) or systems exhibiting severe substrate/product inhibition.

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