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Protein Concentration Calculator
Estimate protein concentration via A280 UV absorbance
Formula: Concentration (mg/mL) = [ $A_{280}$ / ($\varepsilon \times \text{Path Length}$) ] $\times$ Dilution Factor. Default extinction coefficient ($\varepsilon$) for Bovine Serum Albumin (BSA) is approx $0.667\text{ mL}\cdot\text{mg}^{-1}\cdot\text{cm}^{-1}$.
Spectrophotometer Parameters
Calculation Results
⚠️ $A_{280}$ protein quantification can be influenced by nucleic acid contamination, turbidity scattering, and aromatic amino acid composition (Tryptophan and Tyrosine). Ensure blank subtraction is performed correctly and quartz cuvettes are used for UV measurements.