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qPCR Efficiency Calculator
Calculate real-time PCR efficiency and reaction performance from standard curve slope
Formula: Efficiency ($E$) = $(10^{-1/\text{slope}} - 1) \times 100\%$. An ideal 100% efficient amplification yields a slope of $-3.32$ with an $R^2$ value close to $1.00$.
Standard Curve Parameters
Calculation Results
⚠️ qPCR efficiency should ideally fall between 90% and 110% (slope between $-3.10$ and $-3.58$) with an $R^2 \ge 0.98$. Sub-optimal efficiencies can be caused by pipetting errors, PCR inhibitors, poor primer design, or degraded reagents. Always verify melt curves for single peak amplification.